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Image Search Results
Journal: Applied and Environmental Microbiology
Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
doi: 10.1128/AEM.00212-15
Figure Lengend Snippet: (A) PFGE macrorestriction patterns of Streptococcus hyointestinalis strains restricted with SmaI. Lane 1, S. hyointestinalis DPC6484; lane 2, S. hyointestinalis LMG14579; lane 3, S. hyointestinalis LMG14581; lane 4, S. hyointestinalis LMG14582; lane 5, S. hyointestinalis LMG14583; lane 6, S. hyointestinalis LMG14585; lane 7, S. hyointestinalis LMG14586; lane 8, S. hyointestinalis LMG14587. (B) (i) PCR amplification of strains of S. hyointestinalis template DNA with nshT-, nshH-, nshF-, and nshR-specific primers. Lanes correspond to those in panel A. (ii) Comparison of antimicrobial activities of S. hyointesinalis strains DPC6484 (H), LMG14579, LMG14581, LMG14582, LMG14583, LMG14585, LMG14586, and LMG14587 against the indicator strain, L. delbrueckii subsp. bulgaricus LMG6901. Wells are labeled with H (for nisin H) and with the last two digits of the strain designation for the other strains.
Article Snippet: Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( 34 ) using SmaI restriction endonucleases and
Techniques: Amplification, Labeling